rat osteogenic sarcoma cell line  (ATCC)

Journal:

Article Title: Proviral insertions induce the expression of bone-specific isoforms of PEBP2?A (CBFA1): Evidence for a new myc collaborating oncogene

doi:

Figure Lengend Snippet: Electrophoretic mobility shift analysis of core binding factor activity in cell extracts. Nuclear extracts were derived from T47i lymphoma cells (lanes 2–5), UMR 106 rat osteogenic sarcoma cells (lanes 6–9), or E86 mouse fibroblasts (lanes 10–13). No extract was added to lane 1. All reactions included nonspecific competitor poly-dI-dC, whereas those in lanes 3, 7, and 11 included an excess (100×) of unlabeled target oligonucleotide. Antiserum to the C terminus of the G1 isoform (see Fig. ​Fig.2)2) was added in lanes 4, 8, and 12, along with an excess of the corresponding glutathione S-transferase fusion protein in lanes 5, 9, and 13. The letters a and b indicate the major bandshift and supershift complexes observed with the T47i and UMR106 extracts.

Article Snippet: Northern blot analysis with the til -1E probe revealed faint signals at 5.5 kb from UMR106, a rat osteogenic sarcoma line (ATCC CRL-1661) (not shown).

Techniques: Electrophoretic Mobility Shift Assay, Binding Assay, Activity Assay, Derivative Assay